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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 29;187(3):1091–1095. doi: 10.4049/jimmunol.1100853

Figure 4.

Figure 4

Reduced expression of IL-21 in TFH cells primed in CD11c/Aβb mice. (A) Expression of IL-21 transcript in FACS sorted OT-II TFH generated in WT or CD11c/Aβb mice at d7 p.i. normalized to naïve OTII cells. Results are representative of 3 experiments. (B) Relative expression of Bcl6 and IL-21 in OTII cells polarized towards TFH lineage using splenic CD11c+ or CD23+ cells as APCs compared to Th0 condition. Results are representative of 2 experiments. (C) QPCR analysis of Bcl6 and Blimp1 on CD23+ cells stimulated with F(ab)2 fragments of αIgM alone or in combination with αCD40 with or without IL-21 for 3 days. Results pooled from 3 experiments.