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. 1999 Dec 1;13(23):3081–3091. doi: 10.1101/gad.13.23.3081

Figure 2.

Figure 2

Figure 2

Figure 2

Osmotic induction of proP P1. (A) β-Galactosidase activities programmed by a proP–lacZ protein fusion in rich media during different levels of osmotic induction. RJ4373 was grown in LBN, and NaCl was added to a final concentration of 0.3–0.7 m as shown. The absence of Fis in RJ4373 insures that transcription initiates from the P1 promoter only. β-Galactosidase activities (▴) and cell growth (OD600, ●) were measured at the indicated times after addition of NaCl. β-Galactosidase activities are expressed as Miller units without normalization to cell densities (units per ml culture) to reflect synthesis rates. (B) Primer extension analysis of proP P1 RNA after addition of 0.5 m NaCl to CAG4000 growing in LBN. Times (min) after addition of NaCl (+) or LBN (−) are given at top. The sequence ladder was generated using the same primer used for the primer extension of the RNA. (C) Osmotic induction of proP P1 in minimal media. One-tenth volume of M9 plus glycerol containing 4 m or no NaCl was added to cultures of CAG4000 growing in M9 plus glycerol with or without 1 mm glycine betaine. RNA was extracted from cells collected at the indicated times after salt addition and subjected to primer extension as in B. The two panels represent images taken from one gel at the same exposure and thus are directly comparable.