Figure 6.

Kinetics of DNA binding by CRP after osmotic upshift. (A) In vivo DMS footprinting of cells growing in LBN immediately prior to time = 0 or at various times after NaCl addition as denoted. The locations of the guanines that are diagnostic for CRP binding are shown by the asterisks. (B) Graph depicting the occupancy of the CRP site at different times after osmotic upshift. The level of protection immediately preceding the osmotic upshift (time 0) was set at 100% occupancy and the amount of reactivity at 15 min was set at 0% occupancy. (C) Binding to the isolated proP CRP-binding site. In vivo DMS footprinting was performed on cells carrying pRJ1662 immediately prior to time 0, 10 min, or 30 min after addition of 0.6 m NaCl. (D) Binding to the CRP site in the galP1Δ4 promoter. Cells containing pRJ1663 were treated as in C.