Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Osmotic sensitivity of CRP-mediated activation of the lac promoter. Primer extension analysis of transcription initiated at the lac promoter by the CRP-dependent wild-type lac promoter (RJ3354) or the CRP-independent lacL8UV5 promoter (RJ3355). Cells were grown in LBN + IPTG and 0.5 m NaCl was added to a portion of the culture. RNA was extracted 10 min and 30 min after salt addition and subjected to primer extension.
