Fig. 5.

Number of mRNAs whose abundance in the polysome fraction changed after treatment in wild-type livers. GCN2 or PERK mouse livers were perfused for 35 min with perfusate ± Met or ± tBuHQ, respectively. Total RNA was extracted from the unfractionated supernatants and polysome fractions for use in hybridization array experiments. A: analyses of the effect of treatment on mRNA abundance in the polysome fraction were done by sorting of the Z scores of genes in the arrays. mRNAs with Z score > 1.5 in polysome fraction of wild-type treated livers were considered to have shifted into polysomes after activation of the eIF2α kinase. mRNAs with Z score less than −1.5 in polysome fraction of wild-type treated livers were considered to have shifted out of polysomes after activation of the eIF2α kinase. B: Venn diagram of mRNAs that shifted into polysomes after activation of GCN2 or PERK. C: Venn diagram of mRNAs that shifted out of polysomes after activation of GCN2 or PERK.