Fig. 6.

Functional categories of genes whose mRNAs shift into or out of polysomes after Met deprivation, in the presence of GCN2. Gcn2+/+ or −/− mouse livers were perfused for 35 min ± Met, and supernatant was fractioned on a 20–47% sucrose density gradient. Total RNA was extracted from the unfractionated supernatant and the polysomal gradient fraction for use in hybridization array experiments. Analyses of GCN2-dependent shift of mRNAs into polysomes were done by sorting of Z scores of genes in arrays containing unfractionated supernatant and polysomal gradient fraction. Sublists of sorted genes were used for biological process classification in GeneSpring. Bars represent number of genes within the functional category whose mRNA shifted into (positive values) or out of (negative values) polysome fraction. A functional category is included only if it contains ≥3 genes and has a GeneSpring P value of <0.05.