Table 2.
Ectopic expression of Xvax1 in the optic vesicle of hedgehog-injected Xenopus embryos
|
|
RNA injected (pg)
|
Cell injected
|
n
|
Xvax1 induction (%)
|
|---|---|---|---|---|
| X-shh | 100 | 2/2 | 94 | 59 |
| 500 | 2/2 | 48 | 67 | |
| 500 | 1/2 | 53 | 72 | |
| X-bhh | 100 | 2/2 | 74 | 58 |
| 500 | 2/2 | 67 | 83 | |
| 500 | 1/2 | 44 | 73 | |
| X-chh | 100 | 2/2 | 69 | 36 |
| 500 | 2/2 | 66 | 62 | |
| 500 | 1/2 | 102 | 52 |
Embryos were injected into one or both cells at the two-cell stage with the RNAs as indicated. At tadpole stage, embryos were scored for ectopic expression of Xvax1 in the eye vesicle; (n) number of embryos examined.