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Cell number of wild-type (A, n = 3) and TSC2-null (B, n = 3) MEFs (day 2) and control (C, n = 5) and LAM cells (D, n = 5) in the presence or absence of doxycycline (0.1–100 µg·mL−1). *Significant difference from unstimulated (BSA), P≤ 0.05 one-way repeated measures anova. BSA, bovine serum albumin; LAM, lymphangioleiomyomatosis; MEF, mouse embryonic fibroblast; TSC, tuberous sclerosis complex.
