Representative gelatin zymogram showing constitutive production of MMP-2 and MMP-9 by LAM and control cells. Cells were incubated in 0.1% BSA for up to 7 days (as indicated). MMP-2 and MMP-9 expression was detected by enzymatic digestion of the gel (white bands) (A). Total MMP-2 expression from unstimulated (B) and FBS-stimulated (C) cells detected using elisa. Mean data ± SEM from control (n = 4) and LAM cells (n = 6–7). *Significant difference from day 3, P≤ 0.05 two-way repeated measures anova. BSA, bovine serum albumin; FBS, fetal bovine serum; LAM, lymphangioleiomyomatosis; MMP, matrix metalloproteinase.