Figure 7.

Inhibiting TXNIP expression prevents death and loss of RGC. (A, B) Representative images and statistical analysis showing that intravitreal injection of NMDA induced extensive RGC death as indicated by about 8-fold increase of TUNEL-labelled cells (arrows) mainly in RGC and inner nuclear layer (INL) of the rat retina compared with NMLA-controls (200 × magnification). (C) Statistical analysis of the number of neuronal cells in the ganglion cell layer (GCL) in retina sections stained with H/E. Intravitreal NMDA injections resulted in loss of 30% of cells in the GCL in the posterior and in the central regions of the retina. Co-treatment with verapamil (V) blocked these effects in NMDA-injected retinas but did not affect NMLA-injected controls. *P < 0.05, significantly different from the other groups..IPL, inner plexiform layer; ONL, outer nuclear layer; ROD, relative optical density.