Skip to main content
. 2011 Jun 13;589(Pt 15):3883–3895. doi: 10.1113/jphysiol.2011.206193

Figure 2.

Figure 2

A, fold difference in muscle mRNA expression of genes thought to control muscle protein degradation (20S proteasome, MAFbx and MuRF1) in critically ill patients (n = 10). Values are expressed as 2−ΔΔCt normalized to endogenous HMBS. Muscle biopsies from healthy, age- and sex-matched controls (n = 10) were used as a calibrator with a value of 1. *, ***Significantly different from control (P < 0.05, P < 0.001). Data represent mean ± SEM. B, fold difference in the relative optical density of 20S proteasome, MAFbx and MuRF1 proteins in critically ill patients (n = 10) quantified by Western blotting. Biopsies from healthy, age- and sex-matched controls (n = 10) were used as a calibrator with a value of 1. **, ***Significantly different from control (P < 0.01, P < 0.001). Data represent mean ± SEM. C, typical Western blots for 20S proteasome, MAFbx and MurF1 proteins in muscle biopsies obtained from controls (n = 10) and critically ill patients (n = 10).