Figure 5.

Drug sensitivity of cell spheroids of BCa alone and BCa/fibroblast mixture. Epithelial BCa PyT cells were mixed with or without MEF cells, a total of 1x10⁴ cells in medium containing 2% matrigel were seeded onto the 96-well precoated with matrigel to form the spheroids (a standard 3D culture). The cells were incubated at 37⁰C for 5-10 days to allow the formation of aggregates/spheroids. After carefully removing the old medium, the spheroids were incubated with medium containing a serial concentration of YC-1. Shown are the images of spheroids of BCa PyT cells alone (upper row) and spheroids of PyT/MEF mixture (1:3, lower row) on 21 days after drug treatment without or with 0.1, 1, 10, and 100 μM YC-1.