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. 1998 Oct 1;12(19):2992–2996. doi: 10.1101/gad.12.19.2992

Figure 1.

Figure 1

Figure 1

A Tat-affinity column depletes a factor(s) required for transcription elongation. Nuclear extract was chromatographed on a Tat-affinity column, or a control column containing the Tat mutant, Tat(K41A). FT fractions were collected, and bound proteins eluted with 0.5 m or 1.0 m KCl. (A) Activities of the nuclear extract input and the FT fractions were tested in a standard RO transcription assay or by primer-extension. The RO transcripts are shown at top; the primer-extension products at bottom. (B) The Tat protein-affinity column eluates (5 or 10 μl) were tested for their ability to complement the Tat protein-affinity column FT to support transcription. RO transcripts and primer-extension products are shown.