Figure 2.

Induction of CA-RhoA by Dox causes albuminuria in mice. (A) Double transgenic mice were treated with Dox or vehicle for 4 weeks, and the urine ACR (μg/mg) was determined before and after the treatment. The ACR in Dox-treated mice (n = 15) was significantly higher than in vehicle-treated mice (n = 9) after 4 weeks of treatment. **P < 0.001 versus −Dox (4 weeks). (B) After 4 weeks of treatment with Dox, 3 μl of urine samples were separated by SDS-PAGE and stained with Coomassie Brilliant Blue. Dox-treated mice showed intense albuminuria.