Figure 1.

Daam1 protein is detectable during Xenopus pronephric development, with Daam1 and WGEF transcripts expressed in the developing pronephros. (A) Immunoblot (IB) of lysates from stage 2 to 8 oocytes and stage 1 to 41 embryos (approximately 1/2 oocyte or embryo per lane), showing Daam1 and GAPDH (loading control) protein from oocyte through tailbud stages, a timeline encompassing pronephric development (stages 12.5 to 41). (B) RT-PCR of pronephric anlagen isolated from stage 12.5 to 35 embryos showing expression of Daam1 and WGEF, with intensification at stage 25 during pronephric differentiation and morphogenesis.