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. 2011 Sep;22(9):1654–1664. doi: 10.1681/ASN.2010101086

Figure 2.

Figure 2.

Daam1 knockdown in Xenopus does not affect expression of early pronephric determination and patterning markers. (A) Single-cell embryos were uninjected, injected with 40 ng of Std MO, or injected with 40 ng Daam1 MO. IB analysis from gastrula-stage embryos (approximately 1 embryo equivalent per lane) shows Daam1 protein levels were reduced in Daam1-MO-injected embryos as compared with uninjected or Std-MO-injected embryos. (B through D) The left V2 blastomere of eight-cell stage embryos was injected with 20 ng of Std MO or Daam1 MO. Embryos developed until stage 29 to 30 and were analyzed for Lim1 or Hnf1β expression levels on their injected (left) versus uninjected (right) sides. (B) The fraction of Daam1-depleted embryos with reduced Lim1 and Hnf1β marker expression on the injected side as compared with the uninjected side was similar to that of Std-MO-injected controls. (C) Injected sides of Daam1-MO and Std-MO-injected embryos do not have reduced Lim1 expression. (D) Injected sides of Daam1-MO- and Std-MO-injected animals do not have reduced Hnf1β expression. (C, D) Bars are 500 μm.