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. 1998 Oct 1;12(19):2997–3007. doi: 10.1101/gad.12.19.2997

Figure 3.

Figure 3

Figure 3

Figure 3

Figure 3

Figure 3

Figure 3

Effects of Raf on cell cycle regulators. Control (Babe) and GFPΔRaf-1:ER ([YY]- and [DD])-expressing IMR-90 cells were rendered quiescent for 24 hr, at which time 4-HT was added to a final concentration of 1 μm. Cell extracts were prepared at different times (2–48 hr) after the addition of 4-HT, and the expression of GFPΔRaf-1:ER (A), p16Ink4a (B), p21Cip1 (C), cyclin D1 (D), p27Kip1 (E), and p42 MAP kinase (F) was assessed by Western blotting with the appropriate antisera as described in Materials and Methods. As a control, an equivalent volume of ethanol was added to the cells for 48 hr (48−).