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. 2011 Sep;22(9):1696–1706. doi: 10.1681/ASN.2010070728

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Copyright © 2011 by the American Society of Nephrology

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Figure 4. — OxATP reduces cell proliferation of pronephric kidneys in pkd2 morphants. Embryos at 5 dpf were stained for anti-PH3 (red) and anti-α6F (green). Images were merged for counting proliferating cells (orange) in the pronephric kidneys. (A) A wild-type sibling at 5 dpf showing a proliferative cell in the pronephric kidney (arrow). (B) A pkd2 morphant with increased proliferating cells in the pronephric kidney (arrows). (C) A BzATP-treated pkd2 morphant showing multiple proliferating cells in the pronephric kidney (arrows). (D) Reduced proliferating cells in an OxATP-treated pkd2 morphant embryo (arrow). (E) Counts for anti-PH3 and anti-α6F positive cells in the pronephric kidneys. n = 16 to 26 for each group. * indicates significant different means compared with the sibling controls with P < 0.05 by Kruskal-Wallis test and Dunn's multiple comparison test.