Figure 5.

Coinjection of morpholinos against pkd2 and p2rx7 in zebrafish embryos inhibits the cystic phenotype. (A) Note the cystic dilation (arrow) of the pronephros in pkd2 morphants were rescued by p2rx7 knockdown. The glomerular structure is circled in white. (B) Reduced frequencies of cystic phenotype in pkd2 morphants with microinjection of morpholinos against p2rx7 at the ATG site or the splicing sites of exon 2 and exon 14. *P < 0.01. n = 43 to 83 in each groups. (C) RT- PCR from lysates of 48-hpf wild-type and pkd2 morphant embryos coinjected with the splicing-morpholinos targeted against p2rx7 exon 2 and exon 14, showing the splicing is blocked in the p2rx7 transcripts. P2rx7 was amplified using primers directed to exon 1 to 13 (818 bp) and exon 9 to 14 (538 bp).