Table 3.
Solutes identified by LC/MS
| Solute Name or Mass (D) | Colectomy/with Colon | Hemodialysis/ Normal | Dialytic Reduction Ratio |
|---|---|---|---|
| Colon-derived uremic solutes identified by analysis of chemical standards | |||
| PCSa | 0.01 | 11 | 0.25 ± 0.02 |
| α-N-phenylacetyl-l-glutamineb | 0.07 | 91 | 0.73 ± 0.09 |
| ISb | 0.02 | 23 | 0.31 ± 0.03 |
| indoxyl glucuronidec | 0.02 | e | 0.79 ± 0.06 |
| 5-hydroxyindolec | e | e | 0.83 ± 0.08 |
| Colon-derived uremic solutes not identified by analysis of chemical standards | |||
| 201.0786a | 0.03 | e | 0.10 ± 0.26 |
| 204.0092a | e | e | 0.51 ± 0.15 |
| 229.0043a | e | e | 0.70 ± 0.07 |
| 230.0689a | e | e | 0.07 ± 0.10 |
| 284.0813a | e | e | e |
| 284.0886a | e | e | 0.77 ± 0.06 |
| 301.1058a | e | e | 0.32 ± 0.11 |
| 301.1156a | e | e | 0.83 ± 0.04 |
| 306.0407a | e | e | 0.81 ± 0.07 |
| 306.0709a | e | e | 0.82 ± 0.03 |
| 325.0797a | e | e | 0.81 ± 0.05 |
| 334.1921a | e | 23 | 0.18 ± 0.07 |
| 376.1339a | 0.32 | e | 0.82 ± 0.03 |
| 416.1321a | e | e | 0.80 ± 0.04 |
| 420.1599a | 0.26 | e | 0.82 ± 0.03 |
| 474.1527a | e | 4 | 0.62 ± 0.06 |
| 641.3412a | e | e | 0.73 ± 0.07 |
| 169.0525b | e | e | 0.42 ± 0.09 |
| 229.0045b | e | e | 0.71 ± 0.07 |
| 244.0847b | e | e | 0.26 ± 0.18 |
| 275.0096b | 0.02 | e | 0.70 ± 0.06 |
| 276.0302b | 0.05 | e | 0.79 ± 0.05 |
| 101.0105c | 0.19 | e | 0.67 ± 0.06 |
| 192.0552c | 0.21 | 39 | 0.39 ± 0.25 |
| 195.0529c | 0.02 | 38 | 0.71 ± 0.07 |
| 265.0949c | 0.06 | e | 0.79 ± 0.06 |
| 283.0839c | 0.04 | e | 0.73 ± 0.20 |
| 350.1870c | 0.03 | e | 0.85 ± 0.02 |
| Colon-derived solutes that do not accumulate in uremia | |||
| 3-indolepropionic acida | e | 1 | −0.07 ± 0.05 |
| 129.0578b | 0.04 | 1 | −0.04 ± 0.08 |
| Additional indole and phenyl compounds identified by analysis of chemical standards | |||
| phenyl glucuronided | 0.15 | e | 0.80 ± 0.06 |
| cinnamoylglycined | 0.19 | 14 | 0.22 ± 0.18 |
| HIPPd | 0.28 | 27 | 0.60 ± 0.06 |
| indoleacetic acidd | 0.57 | 5 | 0.48 ± 0.08 |
| 2-hydroxybenzoic acid (salicylic acid)d | 0.68 | 5 | 0.46 ± 0.24 |
| indolelactic acidd | 1.8 | 3 | 0.12 ± 0.09 |
| l-kynurenined | 2.0 | 2 | 0.44 ± 0.07 |
| Found in higher concentration in patients without colons | |||
| 331.3233b | 2.9 | 2 | 0.30 ± 0.35 |
Mass values are for neutral compounds assuming monovalent ion detection by MS. Colectomy/with Colon, estimated ratio of average concentration in hemodialysis patients without colons relative to hemodialysis patients with colons; Hemodialysis/Normal, estimated ratio of average concentration in hemodialysis patients with colons relative to normal subjects; Dialytic Reduction Ratio, mean ± SD fractional reduction in plasma levels achieved by dialysis treatment in patients with colons. Levels were too low to estimate a reduction ratio for one solute.
Difference between patients with and without colons: asolute not detectable in patients without colons;
bP < 0.05;
cP < 0.10;
dP > 0.4.
eConcentrations were too low to be estimated in dialysis patients without colons (for Colectomy/with Colon) or in normal subjects (for Hemodialysis/Normal).