Table 1.
Summary of the products of chromosome VII-L excision events
Strain
|
Sequence
|
Distance from telomere (kb)
|
No. of samples
|
Tract length (bp)
|
|||
---|---|---|---|---|---|---|---|
100–200
|
200–300
|
300–400
|
400–500
|
||||
YJS231 | C1–3A | 5 | 20 | 10% | 45% | 40% | 5% |
YJS2 | C1–3A | 20 | 16 | 6% | 69% | 25% | 0 |
YJS331 | C1–3A | 200 | 5 | 0 | 60% | 40% | 0 |
YJS233 | C4A2 | 5 | 10 | 40% | 10% | 40% | 10% |
YJS1 | C4A2 | 20 | 19 | 0 | 47% | 53% | 0 |
YJS357 | C4A2 | 200 | 10 | 30% | 0 | 30% | 40% |
YJS369 | Unique | 5 | 10 | 0 | 100% | 0 | 0 |
YJS46 | Unique | 20 | 10 | 0 | 100% | 0 | 0 |
YJS367 | Unique | 200 | 8 | 0 | 100% | 0 | 0 |
Data are displayed as the percentage of excision events that resulted in tract lengths of the designated size. The approximate length was determined by either genomic Southern analysis for strains YJS2, YJS233, YJS1, YJS357, YJS46 or colony PCR for strains YJS231, YJS2, YJS357, YJS369, YJS367 (Materials and Methods).