Table 2.
Effects of RAD52 and RAD1 on recombination between direct repeats
| Strain
|
Distance from telomere (kb)
|
Tract sequence
|
RAD allele
|
Rate (s.d.)a
|
radΔ/RAD
|
|---|---|---|---|---|---|
| YJS231 | 5 | C1–3A/TG1–3 | wild type | 2.9 × 10−7 (±0.80) | — |
| YJS288 | 5 | C1–3A/TG1–3 | rad52Δ | 3.0 × 10−7 (±0.68) | 1.0 |
| YJS393 | 5 | C1–3A/TG1–3 | rad1Δ | 1.8 × 10−8 (±0.75) | 0.06 |
| YJS399 | 5 | C1–3A/TG1–3 | rad52Δrad1Δ | <1.8 × 10−8* | <0.06 |
| YJS233 | 5 | C4A2/T2G4 | wild type | 2.1 × 10−5 (±0.30) | — |
| YJS290 | 5 | C4A2/T2G4 | rad52Δ | 2.6 × 10−6 (±0.66) | 0.1 |
| YJS392 | 5 | C4A2/T2G4 | rad1Δ | 1.2 × 10−5 (±0.17) | 0.6 |
| YJS369 | 5 | unique | wild type | 2.5 × 10−5 (±0.20) | — |
| YJS292 | 5 | unique | rad52Δ | <1.0 × 10−7 | <0.01 |
| YJS2 | 20 | C1–3A/TG1–3 | wild type | 4.4 × 10−6 (±0.70) | — |
| YJS142 | 20 | C1–3A/TG1–3 | rad52Δ | 1.7 × 10−6 (±0.42) | 0.4 |
| YJS146 | 20 | C1–3A/TG1–3 | rad1Δ | 9.0 × 10−7 (±2.40) | 0.2 |
| YJS402 | 20 | C1–3A/TG1–3 | rad52Δrad1Δ | <1.0 × 10−7* | <0.02 |
| YJS1 | 20 | C4A2/T2G4 | wild type | 3.6 × 10−5 (±0.30) | — |
| YJS90 | 20 | C4A2/T2G4 | rad52Δ | 1.6 × 10−5 (±0.18) | 0.4 |
| YJS390 | 20 | C4A2/T2G4 | rad1Δ | 5.8 × 10−6 (±1.10) | 0.16 |
| YJS46 | 20 | unique | wild type | 7.3 × 10−6 (±1.00) | — |
| YJS88 | 20 | unique | rad52Δ | 3.5 × 10−7 (±1.10) | 0.05 |
The rate of FOARLys+ colonies was measured and calculated as described in Materials and Methods.
a
Two (*) 10-colony fluctuation tests on the rad52Δrad1Δ strains at 5 kb from the telomeres resulted in 5/10 and 7/10 colonies for which 0 recombination events were recovered. Similarly for rad52rad1 strains 20 kb from the telomere fluctuation assays resulted in 7/10 and 3/10 colonies in which 0 recombination events were recovered. These fluctuation tests resulted in a median value of zero and are shown as rates less than a maximum value.