Figure 1.

TGF-β-regulated growth of BAH-ER3 cells in the presence of drug selection. On day 0, BAH-ER3 cells were seeded at a density of 5 × 10⁴ cells/well in a six-well plate in DMEM containing 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin. On day 1, the cells were switched to medium with 1× HAT or 6-TG (30 μg/ml) with or without 200 pm TGF-β as indicated. On day 9, the growing cells were stained with crystal violet. (TRE) Phorbol ester TPA response element; (gpt) guanosine phosphoribosyl transferase.