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. 1998 Oct 1;12(19):3084–3095. doi: 10.1101/gad.12.19.3084

Figure 9.

Figure 9

Both the Smad3–Smad4 binding site and the TFE3 binding site are essential for TGF-β-induced activation of the 36 nucleotide PE2.1 element of the PAI-1 promoter. (A) The sequences of one of the two identical repeats of the wild-type and two mutant PE2.1 elements. (B) Lysate from cells overexpressing TβRI-T204D, Smad3, and Smad4 as described in Fig. 7 were incubated with radiolabeled PE2.1 DNA alone (lane 2) or in the presence of unlabeled PE2.1 (lane 3) or mutant PE2.1Sm oligonucleotides (lane 4), followed by gel electrophoresis. Both the radiolabeled DNA and the competing DNA contained two tandem repeats of the corresponding sequence. (C) Luciferase reporter genes driven by two tandem repeats of either the PE2.1, PE2.1Sm, or PE2.1 Em elements were transfected into Hep G2 cells, and luciferase assays were carried out as described in Materials and Methods. (█) With TGF-β; (□) without TGF-β.