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. Author manuscript; available in PMC: 2011 Sep 13.
Published in final edited form as: Mol Ther. 2007 Mar 13;15(6):1211–1219. doi: 10.1038/sj.mt.6300121

Figure 2. Flow cytometry analysis of interferon-γ (IFN-γ)-secreting CD4+ T cells in vaccinated mice.

Figure 2

C57BL/6 mice (five per group) were immunized twice with 2 μg per mouse of Ii DNA, Sig/PADRE DNA, Sig/PADRE/LAMP-1 DNA, or Ii-PADRE DNA, with a 1-week interval. Splenocytes from vaccinated mice were harvested 1 week after the second vaccination and stimulated overnight with the Pan HLA-DR reactive epitope (PADRE) peptide. (a) Representative figure of the flow cytometry data. The numbers in the upper right corner represent the number of IFN-γ-secreting CD4+ T cells per 3 × 105 splenocytes acquired. (b) Bar graph depicting the number of PADRE-specific CD4+ T cells per 3 × 105 splenocytes (means ± SE). The data presented in this figure are from one representative experiment of two performed.