Figure 4. In vivo tumor protection experiments.

C57BL/6 mice (five per group) were immunized twice using a gene gun with 2 μg per mouse of Ii DNA, Ii–Pan HLA-DR reactive epitope (PADRE) DNA, Ii DNA plus SCT-E6 DNA, or Ii-PADRE DNA plus SCT-E6 DNA, with a 1-week interval. One week after the second vaccination, the vaccinated mice were challenged subcutaneously with 5 × 10⁴ TC-1 cells per mouse. The mice were monitored for evidence of tumor growth by inspection and palpation twice a week. The data shown here are from one representative experiment of two performed.