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. Author manuscript; available in PMC: 2011 Sep 13.
Published in final edited form as: Mol Ther. 2007 Mar 13;15(6):1211–1219. doi: 10.1038/sj.mt.6300121

Figure 5. Characterization of E7-specific interferon-γ (IFN-γ)-secreting CD8+ T cells and Pan HLA-DR reactive epitope (PADRE)-specific CD4+ T cells by flow cytometry analysis in vaccinated mice.

Figure 5

C57BL/6 mice (five per group) were immunized twice intradermally using a gene gun with 2 μg per mouse of Ii DNA plus CRT-E7 DNA or Ii-PADRE DNA plus CRT-E7 DNA, with a 1-week interval. Splenocytes from vaccinated mice were harvested 1 week after the second vaccination and stimulated with E7 peptide or PADRE peptide. Splenocytes without peptide stimulation were used as a negative control. The splenocytes were stained for both CD8 and intracellular IFN-γ. (a, b) Representative figures of the flow cytometry data. The numbers in the upper right corner represent the number of (a) E7-specific IFN-γ-secreting CD8+ T cells or (b) PADRE-specific CD4+ T cells per 3 × 105 splenocytes acquired. (c, d) Bar graphs depicting the number of (c) E7-specific T-cells or (d) PADRE-specific CD4+ T cells per 3 × 105 splenocytes (means ± SE). The data presented in this figure are from one representative experiment of two performed.

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