Table 1. Oligonucleotide primers used for generation of individual domain constructs of αA- and αB-crystallins using PCR-based deletion.

Mutant constructs	Direction	Primers (5′-3′)
αA NTD	Fwd	CACCATGGACGTGACCATCCAGCACCCC
	Rev	TTACTCAGAGATGCCGGAGTCCAGCACGGT
αA CD	Fwd	CACCGTTCGATCCGACCGGGACAAGTTCGTC
	Rev	TTAACAGAAGGTCAGCATGCCATCGGCAGA
αA CTE	Fwd	CACCGGCCCCAAGATCCAGACTGGCCTGGAT
	Rev	TTAGGACGAGGGAGCCGAGGTGGGGTTCTC
αB NTD	Fwd	CACCATGGACATCGCCATCCACCACCCC
	Rev	TTATGAGAGTCCAGTGTCAAACCAGCT
αB CD	Fwd	CACCGAGATGCGCCTGGAAAAGGACAGG
	Rev	TTAATTCACAGTGAGGACCCCATCAGA
αB CTE	Fwd	CACCGGACCAAGGAAAGAGGTCTCTGGC
	Rev	CTATTTCTTGGGGGCTGCGGTGAC