Table 3. Secondary structural content of WT αA, WT αB and their individual domain constructs. Percentages were determined by analysis of the Far-UV spectra (Figure 3) using the SELCON3 analysis program.
| Crystallin species | α-Helix (±1%) | β-Sheet (±1%) | β-Turn (±1%) | Random coil (±1%) |
|---|---|---|---|---|
| WT αA |
21.6 |
46.6 |
13.4 |
17.6 |
| αA NTD |
80.5 |
7.9 |
3.2 |
8.4 |
| αA CD |
37.9 |
42.7 |
10.7 |
9.6 |
| WT αB |
19.3 |
48.7 |
12.4 |
19.6 |
| αB NTD |
29.9 |
41.3 |
9.1 |
19.4 |
| αB CD |
20.6 |
48.1 |
14.3 |
18.7 |
| αB CTE | 5.9 | 66.6 | 4.3 | 23.5 |