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. 1999 Dec 15;13(24):3231–3243. doi: 10.1101/gad.13.24.3231

Figure 2.

Figure 2

Dach2 is expressed dynamically during somite development and in myoblast precursors migrating into the limb buds. (A–E,G–I) Whole-mount RNA in situ hybridization: (A) stage (st) 12 and (B) stage 14, arrows point to the newly formed somite I and the more mature somite VI; (C) stage 17; (D) stage 20; (E) stage 23; (G) stage 18 forelimb; (H) stage 20 forelimb; (I) stage 23 forelimb. Arrows in G–I point to myoblast precursors migrating into the forelimbs. (F) Section in situ hybridization on a transverse section of a stage 11 embryo at the level of somite I. (J–L) Transverse section through a stage 20 forelimb visualized for Dach2 RNA distribution, seen as blue (J), and Pax7 protein distribution, seen as red (I). The two images are merged in K, and cells expressing Dach2 and Pax7 overlap and are purple. Red blood cells autoflouresce, and some examples are indicated above and below the asterix (*) in K and L.