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. 1999 Dec 15;13(24):3231–3243. doi: 10.1101/gad.13.24.3231

Figure 3.

Figure 3

Expression patterns of Dach2, Pax3, Eya2, and Six1 overlap in the developing somite and in limb muscle precursors. (A–H) Nonradioactive section in situ hybridizations on adjacent sections: (A–D) stage 11, adjacent transverse sections at the level of somite V; (E–H) stage 20, adjacent transverse sections at the anterior edge of the forelimb. Arrows in E–H point to the hypaxial myoblast precursors at the lateral edge of dermamyotome. (Insets) Magnifications of the dermamyotome (DM) and myotomal (M) layers of sections shown in E–H. (I–L) Whole-mount in situ hybridization of stage 20 embryos, hindlimbs. Arrows point to myoblast precursors migrating into the hindlimbs. (A,E,I) Dach2 expression. (B,F,J) Pax3 expression. (C,G,K) Eya2 expression, Eya2 probe gives weaker signals relative to other probes used. (D,H,L) Six1 expression. Arrowhead in L points to an additional domain of Six1 expression in the posterior limb mesenchyme.