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. 1999 Dec 15;13(24):3231–3243. doi: 10.1101/gad.13.24.3231

Figure 5.

Figure 5

Misexpression of Pax3 and Dach2 in somite explant culture reveals a positive regulatory feedback loop between these two genes. (A) Misexpression of Pax3. (Lane 1) Somites cultured alone; (lane 2) somites cultured with Pax3 retrovirus (+). Cultures analyzed by RT–PCR for GAPDH, MyoD, Dach2, Eya2, and Six1. (B) Misexpression of Dach2 retrovirus. (Lane 1) Somites cultured alone; (lane 2) somites cultured with Dach2 retrovirus (+). Cultures analyzed by RT–PCR for GAPDH and Pax3. (+) The addition of a retrovirus to the culture.