Skip to main content
. 1999 Dec 15;13(24):3231–3243. doi: 10.1101/gad.13.24.3231

Figure 7.

Figure 7

Interaction assays to reveal interactions between Dach2 and Eya2, and Eya2 and Six1 proteins. (A) GST pull-down interaction assays. (Lanes 1–4) Analysis of Dach2–Eya2 interactions. GST alone (lane 1), GST–Dach2 full-length protein (lane 2), and GST–Dach2 amino-terminal fragment (N-term) (lane 3) were tested for their ability to pull down radiolabeled Eya2 protein. (Lanes 5–7) Analysis of Eya2–Six1 interactions. GST alone (lane 5) and GST–Eya2 full-length protein (lane 6) were tested for their ability to pull down radiolabeled Six1 protein. Strength of the interactions can be assessed by comparing the amount of protein pulled down in the test lanes (lane 1–3 and 5–6, respectively) to the amount in the 10% input lane (lanes 4 and 7, respectively). (B) Yeast two-hybrid interaction assays. Cotransformants were analyzed for their ability to show lacZ color reaction. Strength of interactions were judged by the amount of time passed before a color reaction was observed: (0) no staining after 4 hr; (+++) staining in <60 min.