Skip to main content
. 1998 Oct 15;12(20):3156–3161. doi: 10.1101/gad.12.20.3156

Figure 3.

Figure 3

In situ hybridization of the nascent forelimb bud in E9.5 mouse embryos. (A–D) Limb bud of wild-type embryo (bar, 500 μm). Diffuse Fgf-10 expression occurs in limb bud mesenchyme (B). Fgfr2b is expressed throughout the epithelium in the developing limb region (C), whereas Fgf-8 is specifically expressed in AER (D). (E–H) Distribution of Fgf-8 mRNA in Fgf-10+/+ (E,F) and Fgf-10−/− (G,H) embryos (bar, 100 μm). Note the absence of an Fgf-8 signal near the presumptive site of limb bud initiation (arrow) in the Fgf-10−/− embryo. Fgf-8 expression in both Fgf-10+/+ and Fgf-10−/− embryos is similar at other sites including the neuroectoderm of the rostral telencephalon (open arrowhead), the junction of the mesencephalon and rhombencephalon (arrowhead), and the pharyngeal epithelium (*). (A,E,G) Stained with eosin plus hematoxylin in A.