Figure 1.

Wbp2 is broadly expressed in larval tissues and is both nuclear and cytoplasmic. (A) Schematic representation of proteins encoded by the wbp2 gene. Two protein products are predicted (337 and 427 amino-acids long) that each contain a GRAM domain and two evolutionarily conserved PY motifs. (B) Wbp2 protein expression assessed by immunoblotting in S2 cells and third instar larval tissues. S2 cells were transfected with vector control (Con), short Wbp2 isoform (Wbp2 – 337) or treated with dsRNA for Wbp2 in the presence or absence of Cellfectin. Tissues assessed were wings, eyes, brains and salivary glands. Wings expressing the wbp2-RNAi 1 transgene under the control of the 32B-Gal4 driver (Wing RNAi) were also assessed. (C) Subcellular localisation of Wbp2 (green) in S2 cells transfected with a Myc-tagged version of the short Wbp2 isoform. Nuclei are marked by DAPI (magenta). (D) The level of Wbp2 knockdown caused by expression of wbp2-RNAi 1 was assessed in vivo. Planar (a and b) and cross (c and d) sections of Wbp2 protein expression (red) in third instar larval wing discs expressing wbp2-RNAi 1 under the control of the en-GAL4 driver. Reduced Wbp2 expression was observed specifically in the posterior compartment of the wing disc, labelled with GFP (green)