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BclI repeats are not incorporated in telomeres of nonfunctional TER1 mutants. Telomeric profiles of DNAs prepared from the ΔTER1 strain expressing TER1–BclI or TER1 deletion alleles, as indicated. Each pair of lanes contains DNA digested with EcoRI (R) and EcoRI plus BclI (R/B) enzymes, respectively. Each passage represents ∼25 doublings after the loss of the pTERWT plasmid. The same filter was probed first with the BclI probe (A) and subsequently with the wild-type (WT) probe (B).
