Figure 1.

Effect of hypoxia–reoxygenation on ΔΨ_m and mitochondrial morphology. (a) Images of two H9c2 cells transfected with mtYFP (green) and loaded with TMRE (red) acquired before (left) and during hypoxia (H) (60 min: left middle) and during reoxygenation (R) (30 min: right middle; 60 min: right). (b) Cumulative results for TMRE fluorescence in +G medium (upper left, n=35) and in −G medium (upper right, n=29) for mitochondrial morphology and distribution (middle left and right, +G medium: n=35, −G medium: n=56) and for the amount of donuts (lower left and right, +G medium: n=35, −G medium: n=29). ^*P<0.001