Figure 1.

Prdx6^KD BEAS-2B cells exhibit decreases of peroxidase and PLA₂ activities. (a) Mock or pSuper.retro vector containing an shRNA specific for Prdx6 was transfected into BEAS-2B cells at different concentrations. The endogenous Prdx6 expression was evaluated. (b) Retroviruses containing an shRNA specific for Prdx6 were produced, as described in Materials and Methods. The cells were infected with the viruses and selected in puromycin-containing media for 30 days. The Prdx6-knockdown efficacy was examined. (c) In all, 1 × 10⁵ cells were plated into six wells and incubated for various times as indicated. At each different time, the cells were harvested and counted under trypan blue. (d) The wt BEAS-2B and Prdx6^KD cells were exposed to different concentrations of H₂O₂, as indicated, for 2 h. The intracellular level of H₂O₂ was analyzed by DCF stain, as described in Materials and Methods. (e) The iPLA₂ activities in wt BEAS-2B and Prdx6^KD cells were measured according to the manufacturer's recommendations, as described in Materials and Methods. Bars represent mean±S.D. from at least three independent experiments. P-values were calculated using t-test versus wt BEAS-2B or Prdx6^KD cells (^*P<0.001 and ^**P<0.05)