Figure 5.

The PLA₂ activity of Prdx6 is essential for TNF-α/CHX-induced apoptosis. The wt Prdx6 and mutant Prdx6 (S32A) were expressed in Prdx6^KD cells. The wt BEAS-2B (a), Prdx6 (b), wt Prdx6-overexpressing Prdx6^KD (c), and mutant Prdx6 (S32A)-overexpressing Prdx6^KD cells (d) were treated with TNF-α/CHX for different times as indicated. Cells were washed with HBSS, stained with PI, as described in Materials and Methods, and analyzed with the FACSCalibur system. The cell-cycle distributions were determined with the Modfit LT 3.0 software. The results are expressed as mean±S.D. for triplicate assays