Figure 7.

The PLA₂ activity of Prdx6 regulates the production of IL-1β in response to proinflammatory stimulation. (a and b) The wt BEAS-2B and Prdx6^KD cells were stimulated with TNF-α, LPS, poly I/C, and IL-1β for 12 h. Supernatants were collected, and then levels of IL-1β (a) and IL-6 (b) were measured, as described in Materials and Methods. The results are expressed as mean±S.D. for triplicate assays. (c) Mock, wt Prdx6, and S32A Prdx6-mutant vectors were transfected into wt BEAS-2B and Prdx6^KD cells. The cells were stimulated with TNF-α, and then the level of IL-1β was measured, as described in Materials and Methods. The results are expressed as mean±S.D. for triplicate assays. (d) Mock, wt Prdx6, and S32A Prdx6-mutant vectors were transfected into wt BEAS-2B and Prdx6^KD cells. The cells were stimulated with LPS, and then the level of IL-1β was measured, as described in Materials and Methods. The results are expressed as mean±S.D. for triplicate assays. (e) Mock, wt Prdx6, and S32A Prdx6-mutant vectors were transfected into wt BEAS-2B and Prdx6^KD cells. The cells were stimulated with poly I/C, and then the level of IL-1β was measured, as described in Materials and Methods. The results are expressed as mean±S.D. for triplicate assays. P-values were calculated using t-test versus wt BEAS-2B cells, mock-trasfected, or wt Prdx6-transfected cells (^*P<0.05 and ^**P<0.001)