Figure 3.

Top: analysis of the presence of the spliced (XBP1s) or uncut (XBP1u) isoform of XBP1 by western blot. Histograms show the mean ratio for each isoform and referred to actin levels in ventral horns of control intact rats (c, empty bars), and of contralateral (gray bars) and ipsilateral (black bars) ventral horns of rats after root avulsion or after distal nerve axotomy. ^*P<0.05 Bottom: microphotographs of transverse sections of the spinal cord of control and axotomized showing the MNs at the ventral horns immunostained with anti-pan-XBP1 colocalizing with neuronal-specific immunostaining for NeuN in both models at different time points. Dotted lines separate white and gray matter at the ventral side of the spinal cord. Bar=200 μm