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. 2011 Apr 8;18(10):1664–1674. doi: 10.1038/cdd.2011.34

Figure 3.

Figure 3

DNA damage-induced p21 expression requires caspase-2. (a) Determination of DEVDase activities in HCT116 wild-type and p21−/− cells that were either left untransfected or transfected with the indicated siRNAs before they were γ-irradiated and cultured for 2 days. (b) Western blot analyses for the status of the indicated proteins in HCT116 wild-type and p21−/− cells that were either left untransfected or transfected with control or caspase-2 siRNAs before they were exposed to γIR or etoposide. Cells were harvested 36 h (etoposide) or 48 h (γIR) after treatment. The blots shown represent results from two independent experiments. (c) Determination of DEVDase activities in HCT116 wild-type cells that were either left untransfected or transfected with control or caspase-2 siRNAs before they were exposed to etoposide. The arbitrary units (AU) shown in panels a and c are the mean of three independent experiments±(S.D.)