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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: Mol Cell. 2011 Jul 22;43(2):180–191. doi: 10.1016/j.molcel.2011.06.017

Fig. 5.

Fig. 5

ATM-regulated histone methylation of SENP2 promoter in response to DNA damage. (A) HEK293 cells were incubated with TNFα (T) or VP16 (V) for 3 h. ChIP was performed using H3K4me2 or IgG control antibody and analyzed by qPCR with specific primers around the indicated SENP2 κB sites. The data are displayed as in Fig. 4B (mean+SEM). *, p<0.01. **, p<0.001. (B) HEK293 cells stably expressing HA-IκBα-S32/36A were analyzed as in (A). **, p<0.001. n.s., not significant. (C) HEK293 cells pretreated with KU55933 for 1 h followed by TNFα (T) or VP16 (V) for 3 h were analyzed as in (A). (D) HEK293 cells were transfected with control or ATM-targeting siRNA and then treated with VP16 (V) for 3 h prior to ChIP analysis using antibodies indicated. The efficiency of ATM knockdown was also evaluated by immunoblotting with ATM antibody. See also Figure S5.