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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: Mol Cell. 2011 Jul 22;43(2):180–191. doi: 10.1016/j.molcel.2011.06.017

Fig. 7.

Fig. 7

SENP2 is required to limit NF-κB-dependent cell survival in response to DNA damage. (A) Wt and Senp2−/− MEFs treated as indicated were analyzed by immunoblotting with indicated antibodies. (B) Senp2−/− MEFs stably reconstituted with SENP2-wt, SENP2-c/s or vector control (Vec) were analyzed as in (A). (C) Wt, Senp2−/− and Senp2−/− stably expressing HA-IκBα-S32/36A (Senp2−/−S32/36A) were exposed to different doses of CPT and cell viability was analyzed as in ‘Experimental Procedures’ and plotted (mean+SEM). (D) MEF cells as in (B) were analyzed as in (C). *, p<0.01. **, p<0.001. (E) A model depicting negative feedback regulation of NF-κB signaling induced by TNFα or DNA damage. See Discussion for details. See also Figure S7.