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. 1998 Oct 15;12(20):3168–3181. doi: 10.1101/gad.12.20.3168

Figure 3.

Figure 3

Figure 3

Expression analysis of the transgene. (A) Total RNA was isolated from wild-type BAT and the 11 indicated A-ZIP/F-1 tissues (interscapular fat is BAT), electrophoresed, blotted, and probed successively for A-ZIP/F and aP2. All lanes contain 10 μg of RNA, except for the interscapular fat, which contains 5 μg. Quantitation by PhosphorImager showed that A-ZIP/F expression in the heart lane is 3% of the level in BAT. Ethidium bromide staining confirmed RNA loading (bottom). (B) Anti-Flag antibody was used to probe expression of the Flag-tagged A-ZIP/F protein in BAT of A-ZIP/F-1 and littermate wild-type mice. (DN) Mobility of the A-ZIP/F molecule. A recombinant standard (20, 5, 2, 0.5, and 0.2 pmole) was run for quantitation. Molecular mass is indicated on the right.