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. 1998 Oct 15;12(20):3226–3235. doi: 10.1101/gad.12.20.3226

Figure 6.

Figure 6

Translation is required in cis. (A) Polysome distribution of the MFA2/MS2 mRNA when bound by tethered Pab1p. The translational status of the MFA2/MS2 mRNA was determined by isolating polysomes from strains expressing either MS2/Pab1p or MS2/MS2. Sample optical trace is at top. Numbers at bottom indicate collected fractions. (B) Decay of MFA2/MS2 mRNA bearing a stem–loop in its 5′ UTR, analyzed by transcriptional pulse chase. The turnover rate of the MFA2/MS2 mRNA with or without the stem–loop in its 5′ UTR was determined in strains expressing (lanes 13–18 and lanes 19–24) or lacking (lanes 1–6 and lanes 7–12) MS2/Pab1p. Time (in min) following transcriptional repression are given at top of each lane; half-lives are presented at bottom. (C) Quantitation of results. Amounts of mRNA were normalized to the level of 18S rRNA, shown at bottom of each lane in B. (█) Vector alone, no stem–loop; (□) vector alone, stem–loop; (•) MS2/Pab1p, no stem–loop; (○) MS2/Pab1p, stem–loop.