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. 2011 Jan 24;192(2):229–242. doi: 10.1083/jcb.201008121

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© 2011 Stringer and Piper

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Figure 3. — Fusing cargo to the catalytic domain from deubiquitinating enzymes blocks ubiquitination and trafficking to the vacuole. (A) Schematic of DUb catalytic domain fusion proteins (top). Localization of the indicated GFP-tagged proteins fused to either enzymatically active or inactive (*) catalytic domains of Ubp7, HSV-1 UL36, and mCMV M48 (bottom). (B) The effect of DUb fusion proteins (Ste3-RFP-Ubp7 or Fur4-RFP-Ubp7) on coexpressed Gap1-GFP or Ste3-GFP. (C) Cycloheximide chase with cells expressing HA-tagged Ste3-GFP-m48* and Ste3-GFP-M48, which carried active and inactive DUb domains, respectively. Aliquots were removed at the indicated times after cycloheximide addition (100 µg/ml) and immunoblotted for HA and PGK. (D) Ste3-GFP-UL36 or Ste3-GFP-ul36* (with an inactive catalytic domain) were expressed in end3Δ cells and immunoblotted with anti-GFP antibodies (left) or localized (right). (E) Localization of Ste3-GFP or Ste3-GFP-Ubp7 in wild type, rcy1Δ cells, and vps4Δ cells . Bars, 5 µm.