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. 2011 Sep 13;9(9):e1001148. doi: 10.1371/journal.pbio.1001148

Figure 7. Cd81 −/− HSCs exhibit low expression of CDK inhibitors and oxidative stress genes during homeostasis and regeneration.

Figure 7

(A) Proliferating Cd81 −/− HSCs (5FU-Day8) express a significantly lower level of p19Arf while quiescent HSCs (those on 5FU-Day0 and 5FU-Day12) do not. (B) The expression of Nr4a2 is comparable in quiescent WT and Cd81 −/− HSCs but not detectable in proliferating HSCs. (C) Cd81 −/− HSCs in homeostasis present a significantly lower expression of p21waf1/cip1 while those in regeneration do not. (D) The expression of p27Kip in both WT and Cd81 −/− quiescent HSCs are comparable, albeit undetectable in proliferating HSCs. (E and F) The expression of Atm and p130, the genes that respond to oxidative stresses, are only detectable in HSCs (250 to 300 cell equivalents) in a later stage of 5FU-regeneration (5FU-Day12). The expression of both Atm and p130 in Cd81 −/− HSCs are significantly lower than those in WT HSCs. (G) On 5FU-Day8, the less primitive hematopoietic progenitors (cKit+Lin−Sca1+, KLS) possess detectable level of Atm and p130 expression, and the expression level of Atm in the Cd81 −/− progenitor cells is significantly lower than that in the WT cells. Mean percentages ± SD are shown (duplicate or triplicate measurement was undertaken with at least two biological replicates, *p<0.05, **p<0.01, ***p<0.005).