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. 2011 Sep 13;5(9):e1306. doi: 10.1371/journal.pntd.0001306

Figure 4. Role of intra cellular ROS, during apoptosis in developing embryos of S. digitata.

Figure 4

[A] Quantitation of intra cellular ROS generated in embryonic stages after 24 hr treatment with pharmacological agents in terms of relative DCF [Dichloro fluorescein] fluorescence. Data points in A represent mean ± SEM, with n = 3. *P<0.05 by Paired t-test versus untreated controls; **P<0.05 by Paired t-test versus untreated controls;*** P<0.05 by Paired t-test versus untreated controls [B] Overlaid histogram revealing inhibition of intra cellular ROS by pre-treatment with 2.5mM [Red] and 5 mM [Violet] concentrations of NAC [N-Acetyl-L-Cystiene] - a scavenger of intracellular ROS in embryonic stages, treated with 10 µM of Curcumin [Blue] is shown. [C],[D],[E],[F],[G] Quantitation of intra cellular ROS [C]; viability [D], mitochondrial membrane potential [E]; cytosolic Cytochrome-c [F] and cleavage of intracellular caspase substrate PARP in embryonic stages treated with Curcumin in presence or absence of 2.5 mM NAC are shown. Data points in C, D, E, F and G represent mean ± SEM, with n = 3. *P<0.05 by Paired t-test; **P<0.05 by Paired t-test and *** P<0.05 by Paired t-test.