Table 1. GMI [Geometric Mean Intensity] of fluorescence for each of the 8 assays for apoptosis in developing embryos of pathogenic nematode S.digitata after Plumbagin treatment.
Assay* | Microfilariae/Larval stage-1 | Eggs/Prelarval embryonic stages | ||||
Control | 10 µM | 50 µM | Control | 10 µM | 50 µM | |
Annexin-V | 7.52 | 12.67 | 22.56 | 27.48 | 85.27 | 105.49 |
CED-3 | 7.97 | 33.91 | 41.70 | 94.08 | 204.26 | 218.97 |
CED-4 | 8.42 | 35.87 | 41.54 | 95.91 | 211.21 | 226.90 |
CED-9 | 10.71 | 32.16 | 37.64 | 108.38 | 204.81 | 214.76 |
Cytochrome-c | 4.52 | 18.85 | 38.32 | 57.36 | 72.62 | 88.71 |
CleavedPARP | 7.55 | 10.33 | 19.50 | 65.57 | 107.25 | 120.64 |
TUNEL | 10.52 | 41.79 | 81.74 | 66.08 | 106.44 | 124.19 |
JC-1** | 983.84 | 420.03 | 256.68 | 1530.26 | 982.16 | 554.77 |
*The values in the table represent the mean of GMI of fluorescence of 3 independent experiments for each assay.
**Mitochondrial Depolarization assay by Mitoscreen-JC-1-Staining- expressed in terms of reduction in GMI of fluorescence.