Table 1. GMI [Geometric Mean Intensity] of fluorescence for each of the 8 assays for apoptosis in developing embryos of pathogenic nematode S.digitata after Plumbagin treatment.

Assay*	Microfilariae/Larval stage-1			Eggs/Prelarval embryonic stages
	Control	10 µM	50 µM	Control	10 µM	50 µM
Annexin-V	7.52	12.67	22.56	27.48	85.27	105.49
CED-3	7.97	33.91	41.70	94.08	204.26	218.97
CED-4	8.42	35.87	41.54	95.91	211.21	226.90
CED-9	10.71	32.16	37.64	108.38	204.81	214.76
Cytochrome-c	4.52	18.85	38.32	57.36	72.62	88.71
CleavedPARP	7.55	10.33	19.50	65.57	107.25	120.64
TUNEL	10.52	41.79	81.74	66.08	106.44	124.19
JC-1**	983.84	420.03	256.68	1530.26	982.16	554.77

*The values in the table represent the mean of GMI of fluorescence of 3 independent experiments for each assay.

**Mitochondrial Depolarization assay by Mitoscreen-JC-1-Staining- expressed in terms of reduction in GMI of fluorescence.